Concerns have been raised about E. coli and the water quality of a Suffolk river.
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Studies by the University of Suffolk and Woodbridge Town Council showed levels of the bacteria in the Deben "way above" government guidelines.
Prof Nic Bury said more research was needed but it was a "marker of other pollutants going into the river".
The Environment Agency (EA) said E. coli could enter rivers from a number of sources.
It said it was a naturally-occurring bacteria found in the guts of all warm-blooded animals and humans.
"It can enter rivers from sources including birds, dogs, cattle, run-off containing animal faeces, and storm overflows," the EA said.
According to government guidelines, good coastal or inland bathing water standard is less than 500 colony forming units per 100ml of water.
Woodbridge Town Council councillor Eamonn O'Nolan said three out of 13 sites they tested along the Deben were "significantly" higher than the standard.